Other

Part:BBa_K4430000:Design

Designed by: Ruyi Shi   Group: iGEM22_GYHS   (2022-09-20)


gp13


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 474
    Illegal BglII site found at 2148
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1326
    Illegal AgeI site found at 597
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1522


Design Notes

Researchers had shown that gp13 binds strongly to the cell surfaces of E. coli O157 strains. In order to separate E. coli O157 from food samples, gp13 was coupled with carboxyl magnetic beads, and then E. coli O157 could be separated from the samples through magnets.


Source

The source of the part will be its sequence which was retrieved from NCBI.

References