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Other
Part:BBa_K4430000:Design
Designed by: Ruyi Shi Group: iGEM22_GYHS (2022-09-20)
gp13
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 474
Illegal BglII site found at 2148 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1326
Illegal AgeI site found at 597 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1522
Design Notes
Researchers had shown that gp13 binds strongly to the cell surfaces of E. coli O157 strains. In order to separate E. coli O157 from food samples, gp13 was coupled with carboxyl magnetic beads, and then E. coli O157 could be separated from the samples through magnets.
Source
The source of the part will be its sequence which was retrieved from NCBI.